Underexpression of LATS1 TSG in colorectal cancer is associated with promoter hypermethylation

Figure 4 Methylation status of large tumor suppressor 1 in relation to the expression ratio and histological staging of cells. Forty-four colorectal cancer cases were classified according to histological examination: G1: Well-differentiated cells, n = 2 (light grey bars), G2: Moderately differentiated cells, n = 11 (grey bars), G3: Poorly differentiated cells, n = 28 (dark grey bars), G4: Undifferentiated cells, n = 3 (black bars). G1 epigenetic hypermethylation (M) (n = 1), G1 absence of hypermethylation (UM) (n = 1), G2 M (n = 3), G2 UM (n = 8), G3 M (n = 20), G3 UM (n = 8), G4 M (n = 1), G4 UM (n = 2). Vertical bars represent the large tumor suppressor 1 (LATS1) fold ratio calibrated to the average C_t of control (ΔΔC_t^LATS1 = ΔC_t^{LATS1, sample} - ΔC_t^{LATS1, control}), error bars: SE. ^aP < 0.05 vs control group; ^cP < 0.05 between subgroups (Mann-Whitney U test).