Underexpression of LATS1 TSG in colorectal cancer is associated with promoter hypermethylation

Figure 1 Large tumor suppressor 1 mRNA levels in colorectal cancer and control colon biopsies. Quantitative polymerase chain reaction results of large tumor suppressor 1 (LATS1) expression in 142 colorectal cancer (CRC) samples compared with 40 colon biopsies of healthy patients. CRC cases were divided according to clinicopathological data: tumor stage - Dukes’ A (n = 27), B (n = 41), C (n = 54), D (n = 20); histological differentiation of tumor cells (G staging): G1 (n = 3), G2 (n = 48), G3 (n = 88), G4 (n = 3). Vertical bars represent LATS1 fold ratio calibrated to the average C_t of control (ΔΔC_t^LATS1 = ΔC_t^{LATS1, sample} - ΔC_t^{LATS1, control}), error bars: SE. ^aP < 0.05 vs control group; ^cP < 0.05 between subgroups (Mann-Whitney U test).