Reversal of multidrug resistance in gastric cancer cells by CDX2 downregulation

Figure 4 Effect of RNA interference-mediated inhibition of CDX2 mRNA and protein expression and downregulation of CDX2 on apoptosis in vivo. A: mRNA expression level of CDX2 was determined by semiquantitative reverse-transcriptase polymerase chain reaction; B: Protein expression level of CDX2 was determined by western blotting; C: Tumor cell apoptosis was assessed by HE staining and TUNEL assay. CDX2 siRNA induced more apoptosis of tumor cells (× 400); D: Relative tumor volume (RTV) of nude mice in each group is presented. Each time point represents the mean RTV for each group. RTV in the SGC7901/DDP + small interfering RNA (siRNA)-CDX2 group was smaller than that in control animals. ^aP < 0.05 for SGC7901/DDP + siRNA-CDX2 cells vs SGC7901/DDP + siRNA-NC cells and SGC7901/DDP cells. Lane 1: SGC7901/DDP + siRNA-CDX2 cells; Lane 2: SGC7901/DDP + siRNA-NC cells; Lane 3: SGC7901/DDP cells. GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.