Effects of rhein on intestinal epithelial tight junction in IgA nephropathy

Figure 4 Reverse transcriptase polymerase chain reaction analysis for zona occludens protein-1 and occludin mRNA in rat ileum. By reverse transcriptase polymerase chain reaction, amplification products of expected size [150 bp for zona occludens protein (ZO)-1 and 170 bp for occludin] were obtained in the ileum in all four groups of rats. β-actin was the housekeeping protein. The levels of occludin and ZO-1 expression in the IgA nephropathy (IgAN) group were lower than in the control group. In the Rhein-treated and Rhein-prevented groups, the levels were higher than in the IgAN group. 1: Control; 2: IgAN; 3: Rhein-treated; 4: Rhein-prevented.