Hugl-1 induces apoptosis in esophageal carcinoma cells both in vitro and in vivo

Figure 1 Transfection with pEZ-M29-Hugl1 increased Hugl-1 expression and inhibited the proliferation in Eca109 cells. A: Fluorescent expression in Eca109 cells (× 200); B: Real-time reverse transcription-polymerase chain reaction data of Hugl-1 mRNA levels following transfection with pEZ-M29-Hugl1 plasmids (group 3), pEZ-M29-eGFP (group 2), or treatment with phosphate buffered saline (PBS) (group 1); C: Western blotting data showing Hugl-1 protein expression levels following pEZ-M29-Hugl1 transfection compared with control groups; D: The effect of Hugl-1 on cell proliferation was assessed by cell counting Kit-8. Results represent mean values of three experiments and are indicated as mean ± SD. ^aP < 0.05 vs the pEZ-M29-eGFP-treated and PBS-treated groups. GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.