Roles of BN52021 in platelet-activating factor pathway in inflammatory MS1 cells

Figure 4 The effect of BN52021 on platelet-activating factor receptor signaling molecules at the protein level under lipopolysaccharide-induced inflammation. The protein level of p-adenylate cyclase (p-AC) (A), G protein-coupled receptor kinases (GRK) (B), p-phospholipase A₂ (p-PLA₂) (C), phospholipase Cβ (PLCβ) (D) and p-p38-mitogen-activated protein kinase (p-p38 MAPK) (E) was up-regulated after lipopolysaccharide (LPS) stimulation vs the blank control (^aP < 0.05). The up-regulation of p-AC, p-p38 MAPK, GRK and PLCβ protein levels was significantly suppressed by BN52021. However, p-PLA₂ and phosphorylated protein tyrosine kinase (p-PTK) protein levels were insignificantly up-regulated after LPS stimulation and were not significantly changed by BN52021 (F). ^cP < 0.05, ^dP < 0.01 vs LPS + dimethyl sulfoxide (DMSO) groups.