Roles of BN52021 in platelet-activating factor pathway in inflammatory MS1 cells

Figure 2 The dose effect of BN52021 on lipopolysaccharide-induced inflammation was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method and Hoechst 33342/propidium iodide staining. MS1 cell activity at A_{490 nm} was significantly decreased 24 h after administration of 10 μg/mL lipopolysaccharide (LPS) vs the control group (^aP < 0.05). Pretreatment with BN52021 for 20 min before incubation with LPS significantly improved the MS1 cell activity at A_{490 nm}vs the group that received LPS treatment only when its concentration reached 50 μmol/L (^cP < 0.05) (A). Pretreatment with 50 μmol/L BN52021 for 20 min before incubation with LPS significantly improved MS1 cell activity vs the LPS + saline group, and the LPS + dimethyl sulfoxide (DMSO) group as determined Hoechst 33342/propidium iodide staining (B, C, D and E). The arrows indicate the apoptosis (short) and necrosis (long) of the cells.