Role of activin A in carbon tetrachloride-induced acute liver injury

Figure 3 Expression of activin A protein in liver of mouse assessed by immunohistochemical staining. A, B: Activin A expression was examined by using anti-activin A antibody in the same liver tissues on day 1 after olive oil treatment; C, D: Activin A expression was examined by using anti-activin A antibody in the same liver tissues on day 1 after carbon tetrachloride (CCl₄) treatment; E, F: Activin A expression was examined by using anti-activin A antibody in the same liver tissues on day 3 after CCl₄ treatment; G, H: The procedural background control staining was represented by using normal mouse immunoglobulin G instead of anti-activin A antibody in livers of olive oil-treated and CCl₄-treated mice. Red arrows represent lesion area and black arrows represent positive staining for activin A. A, C, E: Magnification × 100; B, D, F, G, H: Magnification × 200.