Glycyrrhizic acid attenuates CCl4-induced hepatocyte apoptosis in rats via a p53-mediated pathway

Figure 2 Impact of glycyrrhizic acid treatment on hepatic apoptosis induced by carbon tetrachloride in rats. A: Liver tissue sections from the different groups were subjected to immunohistochemistry to determine the expression level of cleaved caspase-3 (original magnification, × 400); B: Fluorescence microscopy image showing terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick end labeling (TUNEL) stain (dashed arrows), and the same tissue slices were respectively counterstained with 4’6-diamidino-2-phenylindole (DAPI) to localize the nuclei (arrows). Images of combined with DAPI, indicated TUNEL-positive cells (arrow heads) (original magnification, × 200). GA: Glycyrrhizic acid; CCl₄: Carbon tetrachloride.