Interaction of 14-3-3σ with KCMF1 suppresses the proliferation and colony formation of human colon cancer stem cells

Figure 5 Expression and interaction of 14-3-3, potassium channel modulatory factor 1, quinone oxidoreductase, hydroxyisobutyrate dehydrogenase and 14-3-3plasmids in HEK 293FT cells. A: Expression of the 14-3-3σ, potassium channel modulatory factor 1 (KCMF1), quinone oxidoreductase (NQO2), hydroxyisobutyrate dehydrogenase (HIBADH) plasmids. HEK 293FT cells were transfected with different plasmids. Forty-eight hours post-transfection, the cells were lysed, and Western blotting analysis was performed with an anti-Myc or anti-Flag antibody (1:1000); B: Interaction of 14-3-3σ and KCMF1, NQO2 or HIBADH. HEK 293FT cells were transfected with different plasmids. Forty-eight hours post-transfection, the cells were lysed and immunoprecipitations were performed with an anti-Myc antibody (1:2000). After the proteins were resolved by SDS-PAGE, immunoblot analysis was performed with antibodies against Flag protein (1:2000). 1: Sample 1 KCMF1; 2: Negative Control-1; 3: Sample 2 NQO2; 4: Negative Control-2; 5: Sample 3 HIBADH; 6: Negative Control-3; 7: Negative Control-4; 8: Positive.