Bone-marrow mesenchymal stem cells reduce rat intestinal ischemia-reperfusion injury, ZO-1 downregulation and tight junction disruption via a TNF-α-regulated mechanism

Figure 8 Bone-marrow mesenchymal stem cells and tumor necrosis factor-α blockade attenuate zona occludens 1 downregulation after intestinal ischemia/reperfusion injury. Representative western blots and quantification of zona occludens 1 (ZO-1) protein expression in the intestinal mucosa. Actin was used as a loading control. Values are the mean ± SD (n = 3 rats per group); ^bP < 0.01 vs Sham group; ^dP < 0.01 vs the saline (NS) + ischemia/reperfusion (I/R) injury group (one-way analysis of variance followed by the LSD test). BM MSCs: Bone-marrow mesenchymal stem cells; Anti-TNF: Anti-tumor necrosis factor.