Thinking outside the liver: Induced pluripotent stem cells for hepatic applications

Figure 3 Human induced pluripotent stem cells generated from human foreskin fibroblasts using single lentiviral stem cell cassette kit (Millipore, United States) method. A, B: Human induced pluripotent stem cell (hiPSC) colonies resembling embryonic stem cells in morphology were observed, and iPSC with a flat, packed, tight colony morphology and a high nucleus to cytoplasm ratio (A, × 40) were positive for Oct4 marker on immunocytochemistry (B, × 200); C, D: hiPSCs were differentiated into hepatocytes. At day 13, these differentiated cells exhibited polygonal morphology (C, × 400) and showed pink color (glycogen storage) on periodic acid schiff staining (D, × 200).