Mechanisms of trichostatin A inhibiting AGS proliferation and identification of lysine-acetylated proteins

Figure 8 Identification of the effectiveness of lysine-acetylated antibodies enriching acetylated proteins. Line 1: 20 μg total protein from AGS cells unexposed to trichostatin A (TSA); Line 2: 20 μg total protein from AGS cells exposed to 0.5 μmol/L TSA; Line 3: 20 μg flow-through protein from AGS cells unexposed to TSA, which was incubated with an antibody gel column; Line 4: 20 μg flow-through protein from AGS cells exposed to 0.5 μmol/L TSA, which was incubated with an antibody gel column; Line 5: 100 ng enriched protein from AGS cells unexposed to TSA, which was incubated with an antibody gel column; and Line 6: 100 ng enriched protein from AGS cells exposed to 0.5 μmol/L TSA, which was incubated with an antibody gel column.