Mechanisms of trichostatin A inhibiting AGS proliferation and identification of lysine-acetylated proteins

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Jun 7, 2013 (publication date) through Dec 18, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 7, 2013; 19(21): 3226-3240
Published online Jun 7, 2013. doi: 10.3748/wjg.v19.i21.3226

Table 1 Oligonucleotide sequences used in real-time polymerase chain reaction

Gene	Primer (5' to 3')	Length (bp)
β-actin	F: 5'TGGAGAAAATCTGGCACCA3'	189
β-actin	R: 5'CAGGCGTACAGGGATAGCAC3'	189
p21	F: 5'TCCAAGAGGAAGCCCTAATCC3'	101
p21	R: 5'ACAAACTGAGACTAAGGCAGAAGATG3'	101
p53	F: 5'TCAGTCTACCTCCCGCCATAA3'	231
p53	R: 5'GTGCAGGCCAACTTGTTCAGT3'	231
Bcl-2	F: 5'CCTTTTCTACTTTGCCAGCAAAC3'	149
Bcl-2	R: 5'GAGGCCGTCCCAACCAC3'	149
CDK2	F: 5'GCTAGCAGACTTTGGACTAGCCAG3'	85
CDK2	R: 5'AGCTCGGTACCACAGGGTCA3'	85
CyclinD1	F: 5'AACAGAAGTGCGAGGAGGAG3'	299
CyclinD1	R: 5'CTGGCATTTTGGAGAGGAAG3'	299
Bax	F: 5'CCAGGGTGGTTGGGTGAGACT3'	231
Bax	R: 5'TGGGAGGTCAGCAGGGTAGAT3'	231

Bcl-2: B cell lymphoma-2; CDK2: Cyclin-dependent kinase 2; Bax: Bcl-associated X protein.

Citation: Wang YG, Wang N, Li GM, Fang WL, Wei J, Ma JL, Wang T, Shi M. Mechanisms of trichostatin A inhibiting AGS proliferation and identification of lysine-acetylated proteins. World J Gastroenterol 2013; 19(21): 3226-3240
URL: https://www.wjgnet.com/1007-9327/full/v19/i21/3226.htm
DOI: https://dx.doi.org/10.3748/wjg.v19.i21.3226