Development and application of a real-time polymerase chain reaction method for Campylobacter jejuni detection

doi:10.3748/wjg.v19.i20.3090

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May 28, 2013 (publication date) through Aug 24, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 28, 2013; 19(20): 3090-3095
Published online May 28, 2013. doi: 10.3748/wjg.v19.i20.3090

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Figure 1 Real time polymerase chain reaction standard curve and the linear relationship between Campylobacter jejuni quantity and polymerase chain reaction cycle threshold. A: The standard curve was constructed using genomic DNA from Campylobacter jejuni isolates. Serial dilutions ranging from 100 to 106 CFU/mL of target template were subjected to real time polymerase chain reaction assay; B: The linear relationship between Log CFU/mL and threshold cycles. RFU: Relative fluorescence units.

Citation: Zhang MJ, Qiao B, Xu XB, Zhang JZ. Development and application of a real-time polymerase chain reaction method for Campylobacter jejuni detection. World J Gastroenterol 2013; 19(20): 3090-3095
URL: https://www.wjgnet.com/1007-9327/full/v19/i20/3090.htm
DOI: https://dx.doi.org/10.3748/wjg.v19.i20.3090