Matrix metalloproteinase-9 in the initial injury after hepatectomy in mice

Figure 9 Broad-spectrum matrix metalloproteinase-9 inhibitor GM6001 ameliorate initial injury of the liver 6 h after 80%-partial hepatectomy. We inhibited matrix metalloproteinase-9 by GM6001 in 80%-partial hepatectomy (PH) mice, and determined liver necrosis 6 h after 80%-PH. Liver damage was significantly reduced in the GM6001-treated mice compared with that in the vehicle-treated group (A, B). Serum levels of aspartate aminotransferase (AST) (C), alamine aminotransferase (ALT) (D) and total bilirubin (T-Bil) (E) were significantly lower in the GM6001-treated mice than those in the vehicle-treated mice. Representative images of in situ gelatin zymography analysis with DQ gelatin are shown. Gelatinolytic activity was suppressed in the GM6001-treated mice (G) compared with that in the vehicle-treated mice (F). Representative images of myeloperoxidase staining on remnant liver (RL) sections in vehicle-treated mice (H) and GM6001-treated mice (I) are shown. A histogram of the number of accumulated neutrophils in the RL after extended hepatectomy shows that there are significantly fewer neutrophils in the GM6001-treated mice than in the vehicle-treated mice (J) (^aP < 0.05, ^bP < 0.01 vs vehicle-treated mice).