Matrix metalloproteinase-9 in the initial injury after hepatectomy in mice

Figure 6 Matrix metalloproteinase-9 activity in the remnant liver with wild-type, matrix metalloproteinase-9(-/-), and tissue inhibitor of metalloprote-inase-1(-/-) mice 6 h after 80%-partial hepatectomy is shown. A: Western blotting analysis for matrix metalloproteinase-9 (MMP-9) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) for a loading control was performed. The expression of MMP-9 in wild-type (WT) and tissue inhibitor of metalloproteinase-1 (TIMP-1)(-/-) mice and deletion in MMP-9(-/-) mice were observed at the protein level; B: Gelatin zymography analysis is shown; C-E: Pro-form MMP-9 activity and a slightly active form of MMP-9 were detected in WT and TIMP-1(-/-) mice. The activity of MMP-2 was the same depending on its genotype. In situ gelatin zymography analysis using DQ gelatin for gelatinolytic activity in the liver tissue is shown. A reduction in gelatinolytic activity in MMP-9(-/-) mice and enhancement in TIMP-1(-/-) mice are observed.