Matrix metalloproteinase-9 in the initial injury after hepatectomy in mice

Figure 4 Localization analysis of matrix metalloproteinase-9 by dual immune-fluorescence in the remnant liver after 80%-partial hepatectomy is shown. A: Fluorescent microscopy images of matrix metalloproteinase-9 (MMP-9) labeled in red (Alexa Fluor 568); B: CD68 labeled in green (Alexa Fluor 488); C: A merged image of MMP-9 and CD68 staining are shown; D: MMP-9 labeled in red (Alexa Fluor 568); E: Desmin labeled in green (Alexa Fluor 488); and F: A merged image of MMP-9 and desmin are shown; G: MMP-9 labeled in red (Alexa Fluor 568); H: CD11b labeled in green (Alexa Fluor 488); I: A merged image of MMP-9 and CD11b are shown. Protein expression of MMP-9 was mainly localized in CD11b-positive cells and desmin-positive cells to some degree.