A silybin-phospholipids complex counteracts rat fatty liver degeneration and mitochondrial oxidative changes

Table 3 Concentrations of total glutathione, thioredoxin, nitrosothiols, and activity of glutathione peroxidase in the liver of rat

		Day 14	Day 30	Day 60
Glutathione	S	23.7 ± 1.9a	20.8 ± 2a	22.4 ± 2.4a
33.4 ± 0.8 nmol/mg prt	R	22.3 ± 1.8a	23.9 ± 1.7a	23 ± 1.6a
Thioredoxin	S	9.2 ± 0.9a	8.9 ± 0.8a	4.3 ± 1.0
5.4 ± 0.5 nmol/mg prt	R	7.6 ± 0.7ac	7.8 ± 0.7a	7.4 ± 1.7ac
Nitrosothiols	S	18.1 ± 1.5	19.6 ± 2.5	10 ± 2.3a
20.4 ± 3.1 pmol/mg prt	R	14.4 ± 1.7ac	16.1 ± 2.3a	14.3 ± 1.9ac
GPx	S	4.7 ± 0.6a	6.5 ± 1.3a	4.6 ± 1.6
3.9 ± 0.3 nmol NADH/min/mg prt	R	3.4 ± 0.5c	4.3 ± 1.0c	4.1 ± 1.0

The liver of rats fed a high fat diet (HFD) diet without (S)/with (R) Realsil or a standard control diet (controls). Values from controls are reported in the first column under the parameter. Data are mean ± SD of n = 5 rats per group at each time point.

^aP < 0.05 vs control rats;

^cP < 0.05 vs rats on HFD diet without Realsil at the same time point. GPx: Glutathione peroxidase; NADH: Nicotinamide adenine dinucleotide; prt: Protein.