Inositol-requiring enzyme 1α is required for gut development in Xenopus lavies embryos

Figure 4 Xenopus inositol-requiring enzyme 1α is required for gut development. A: Knockdown of x IRE1α did not change the phenotype and the expression of Xbra and Xsox17α at stage 10.5; B: The inositol-requiring enzyme (IRE) 1α morpholino oligonucleotide (MO)-injected embryos were morphologically normal before the tailbud stages; C: IRE1α knockdown upon injection of 50 ng of MO resulted in a gut defective phenotype. Surgically-resected guts from embryos were shown in C under panel. Coiled structure of gut was not detected in IRE1α MO-injected embryos at stage 43. MO injection was repeated 5 times in a total of 278 embryos.