Inhibiting heme oxygenase-1 attenuates rat liver fibrosis by removing iron accumulation

Figure 6 Perl’s Prussian blue staining, levels of hepcidin, serum and liver iron. A: No iron accumulated in the Sham group; B: A small amount of iron mainly accumulated on Kupffer cells in the bile duct ligation (BDL) group; C: Much more iron accumulation was found in interlobular and macrophagocytes in the cobalt protoporphyrin (CoPP) group; D, F: Almost no iron accumulation was detected in the zinc protoporphyrin (ZnPP) group and deferoxamine (DFX) group; E: Massive iron accumulation was observed in the Fe group; G, H: There were no differences in the hepatic and serum iron content of these six groups; I: Plasma hepcidin also was measured by enzyme-linked immuno sorbent assay (magnification × 400). Values are expressed as mean ± SE (n = 6). ^bP < 0.01 vs Sham group; ^cP < 0.05, ^dP < 0.01 vs BDL group; ^eP < 0.05 vs ZnPP group.