Overexpression of p42.3 promotes cell growth and tumorigenicity in hepatocellular carcinoma

Figure 2 The effect on molecular by overexpression of p42. 3 in HepG2 cells. A: Reverse transcription-polymerase chain reaction and Western blotting were performed to confirm p42.3 overexpression in a stable single colony of HepG2-p42.3-1 and HepG2-p42.3-2 cells. p42.3 expression was deficient in the HepG2-vector control cells. β-actin served as an internal control; B: Expression of proteins shown as mean ± SD. Consistent with p42.3 protein expression, proliferating cell nuclear antigen (PCNA), mitotic arrest deficient 2 (MAD2) and cyclin B1 expression were significantly upregulated. The protein levels of cell division cycle 25 A (Cdc25A) and cell division cycle 25 homolog C (Cdc25C) hardly changed following p42.3 expression (^bP < 0.01 vs HepG2-vector).