Overexpression of p42.3 promotes cell growth and tumorigenicity in hepatocellular carcinoma

Figure 1 Detection of p42. 3 in hepatic cell lines and hepatocellular carcinoma tissues. A: Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that p42.3 was detectable in all 7 cell lines, and the lowest expression was in HepG2 cells; B: Relative expression of p42.3 mRNA in seven hepatic cell lines using quantitative real-time RT-PCR. Data are shown as the mean ± SD, endogenous references was β-actin (^bP < 0.01 vs HepG2); C and D: Expression of p42.3 protein in hepatic cell lines analyzed by Western blotting (C) and shown as mean ± SD (D) (^bP < 0.01 vs HepG2); E: Negative staining of p42.3 in hepatocellular carcinoma-adjacent normal tissue (left), positive staining of p42.3 in tumor (right). Original magnification, × 100; the inset boxes are at original magnification × 200.