Regulation of dipeptidyl peptidase 8 and 9 expression in activated lymphocytes and injured liver

Figure 4 Dipeptidyl peptidase 8 and dipeptidyl peptidase 9 were associated with lymphocyte apoptosis. A: Percentage of annexin V + Raji cells 40 h after transfection with vector, wild type (wt) dipeptidyl peptidase (DPP) 9-V5-His or enzyme-negative mutant (mut) DPP9-V5-His. Annexin V staining was enumerated by flow cytometry; B: Immunoblot of DPP8 and its densitometry (C) immunoblot of DPP9 and its densitometry in Raji cells untreated and treated with dithiothreitol (DTT) or mitomycin C for 24 h. Densitometry are shown as relative to glyceraldehyde 3-phosphate dehydrogenase (GAPDH).