Inhibiting heme oxygenase-1 attenuates rat liver fibrosis by removing iron accumulation

doi:10.3748/wjg.v19.i19.2921

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May 21, 2013 (publication date) through Nov 18, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 21, 2013; 19(19): 2921-2934
Published online May 21, 2013. doi: 10.3748/wjg.v19.i19.2921

Table 1 Primers used for the reverse transcription-polymerase chain reaction and polymerase chain reaction analysis

Gene	Gene ID	Forward/reverse	Sequences 5'-3'	Product size (bp)
HO-1	NM012580	Forward	ATATCTATACGGCCCTGGAA	350
		Reverse	GATGCTCGGGAAGGTGAA
Nrf2	AF304364	Forward	GACGGCAACACTGATTCCA	345
		Reverse	CATCCGCCACTCATTCCT
TGF-β1	NM021578	Forward	CCGCAACAACGCAATCTA	437
		Reverse	TGAGGAGCAGGAAGGGTC
Hepcidin	NM053469	Forward	GCTGCCTGTCTCCTGCTT	159
		Forward	GGTGTCTCGCTTCCTTCG
TIMP-1	NM053819	Forward	CTCTGGCCTCTGGCATCCT	300
		Reverse	ACTCCTCGCTGCGGTTCT
MMP-2	NM031054	Forward	CTGGGCAACAAGTATGAGA	430
		Reverse	CTGTCCGCCAAATAAACC
β-actin	NM031144	Forward	GAGGGAAATCGTGCGTGAC	445
		Reverse	CTGGAAGGTGGACAGTGAG

HO-1: Heme oxygenase-1; Nrf2: Nuclear factor-E2-related factor 2; TGF-β1: transforming growth factor-β1; TIMP-1: Tissue inhibitor of metalloproteinase-1; MMP-2: Matrix metalloproteinase-2.

Citation: Wang QM, Du JL, Duan ZJ, Guo SB, Sun XY, Liu Z. Inhibiting heme oxygenase-1 attenuates rat liver fibrosis by removing iron accumulation. World J Gastroenterol 2013; 19(19): 2921-2934
URL: https://www.wjgnet.com/1007-9327/full/v19/i19/2921.htm
DOI: https://dx.doi.org/10.3748/wjg.v19.i19.2921