MAWBP and MAWD inhibit proliferation and invasion in gastric cancer

Figure 2 Effect of mitogen-activated protein kinase activator with WD40 repeats binding protein and mitogen-activated protein kinase activator with WD40 repeats on proliferation and tumorigenicity of gastric cancer cells. A: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay showed that growth of SGC7901 cells overexpressing mitogen-activated protein kinase activator with WD40 repeats (MAWD)/MAWD binding protein (MAWBP) was markedly inhibited; B: In BGC823 cells with inhibition of expression of MAWBP and MAWD, cell growth was inversely increased; C: In soft agar assay, colonies of cells with overexpression of MAWBP, MAWD and MAWBP/D were reduced in number and size compared with cells transfected with vectors alone (original magnification of clones: ×100); D: Knockdown group demonstrated the opposite effects. There was an increase in the number and size of sh-MAWBP, sh-MAWD, sh-MAWBP/D cells compared with cells transfected with vectors alone; E: Nude mouse xenografts. Tumors induced by MAWBP, MAWD and MAWBP/D cotransfected cells were smaller than those of the vector group; F: Xenograft weight. The average tumor weight were calculated from five nude mice in every group. Data are presented as the mean ± SD from three independent experiments. ^bP < 0.01 vs vector group.