Lipoic acid suppresses portal endotoxemia-induced steatohepatitis and pancreatic inflammation in rats

Figure 5 Histopathologicalexamination of pancreatic islets. A: Tissue samples from each subset of animals were analyzed by hematoxylin and eosin (HE) staining (a-C_PV, b-F_PV, c-F_{PV + LA}, d-F_PLPS, e-F_{PLPS + LA}) and immunostaining with an antibody against CD68 (f-C_PV, g-F_PV, h-F_{PV + LA}, i-F_PLPS, j-F_{PLPS + LA}); This data was then quantitated as (B) percentage of islets infiltrated and (C) number of CD68+ cells per islet. Arrows indicated CD68-positive cells. I: Islet. A total of 100 ± 25 islets per experimental group were blindly scored for CD68-positive cells around the periphery and/or within islets from 5 to 6 different animals. Islet area was measured by the area of islet capsule in pancreatic section with HE stain and computed using AxioVision LE 4.8.2.0 software. Values are expressed as mean ± SE, n = 6 per group. Line bar: 50 μm. ^aP < 0.05 vs C_PV; ^cP < 0.05 vs F_PV; ^eP < 0.05 vs F_PLPS. C: Regular diet; F: High-fructose enriched diet; LA: Lipoic acid; LPS: Lipopolysaccharides.