Establishment of mouse intestinal myofibroblast cell lines

Figure 5 Expression of lipopolysaccharide-related proteins and responses of primary intestinal myofibroblasts, LmcMF, and SmcMF to lipopolysaccharide. A: The expression levels of indicated proteins in primary intestinal myofibroblasts (IMFs) (primary), LmcMF, and SmcMF were investigated by Western blotting; B-D: Primary IMFs (B), LmcMF (C), and SmcMF (D) were stimulated with lipopolysaccharide (LPS) (20 ng/mL) for indicated periods. The IκBα degradation and p38 MAPK phosphorylation were determined by Western blotting. Representative blots from 3-4 independent experiments are shown. Actin was used as a loading control.