Establishment of mouse intestinal myofibroblast cell lines

Figure 4 Immunofluorescence staining for α-smooth muscle actin and vimentin in primary intestinal myofibroblasts, LmcMF, and SmcMF. Primary intestinal myofibroblasts (IMFs), LmcMF, and SmcMF were immunostained with α-smooth muscle actin (α-SMA) (A) and vimentin (B) (red). SYTOX Green was used for nuclear labeling (green). Representative images are shown. Scale bars indicate 40 μm.