Emodin regulating excision repair cross-complementation group 1 through fibroblast growth factor receptor 2 signaling

Figure 5 Comparison of fibroblast growth factor receptor 2, phosphorylated extracellular signal-regulated kinase 1/2, excision repair cross-complementing gene 1 expression in HepG2, HepG2/oxaliplatin and HepG2/oxaliplatin/T detected by Western blotting. A-C: In comparison with in the parental HepG2 cell line, the expression levels of the fibroblast growth factor receptor 2 (FGFR2), phosphorylated extracellular signal-regulated kinase 1/2 (pERK1/2) and excision repair cross-complementing gene 1 (ERCC1) in the resistant cell line HepG2/oxaliplatin (OXA) were significantly increased, however, the pERK1/2 and ERCC1 expression levels in HepG2/OXA/T cells were significantly inhibited, as FGFR2 expression was silenced. The expression levels of pERK1/2 and ERCC1 in the HepG2 cells and that of FGFR2, pERK1/2 and ERCC1 in HepG2/OXA cells, was increased in the OXA + fibroblast growth factor 7 (FGF7) group and decreased in the OXA + emodin group with statistical significance compared with the OXA group, but the trend of those significant differences disappeared in HepG2/OXA/T cells (^aP < 0.05, ^bP < 0.01 vs OXA group; ^cP < 0.05, ^dP < 0.01 vs OXA + FGF7 group); D: Western blotting of FGFR2, pERK1/2, ERCC1 in HepG2, HepG2/oxaliplatin and HepG2/oxaliplatin/T. M: Marker.