Effect of biliary drainage on inducible nitric oxide synthase, CD14 and TGR5 expression in obstructive jaundice rats

Figure 7 Representative immunohistochemical analysis of TGR5 in rat liver tissues of sham operation, obstructive jaundice, internal and external biliary drainage groups. TGR5-immunoreactivity was detected mainly in the plasma membrane of Kupffer cell and its intracellular compartments, and also localized in some sinusoidal endothelial cells and biliary epithelial cells, but merely in hepatocytes. A: There was slight expression of TGR5 in rats of sham operation (SH); B: After bile duct ligation, the TGR5-immunoreactivity became stronger as compared to SH rats; C: After relief of jaundice by internal biliary drainage (ID), the expression of TGR5 was markedly suppressed; D: The expression of TGR5 in external biliary drainage (ED) rats was still at a high level; E: Comparison of mean absorbance values of TGR5 in rat liver tissues among the four groups. OJ: Obstructive jaundice; IHC: Immunohistochemical.