Twist2 is a valuable prognostic biomarker for colorectal cancer

Table 4 Univariate and multivariate analysis of the prognostic factors for disease-free survival

Prognostic factors	Univariate analysis			Multivariate analysis
	HR	95%CI	P value	HR	95%CI	P value
Gender
Female	1
Male	1.001	0.587-1.706	0.998	NA	NA	NA
Age
< 59	1
≥ 59	1.060	0.621-1.809	0.831	NA	NA	NA
T-stage
T1-2	1
T3-4	1.258	0.833-1.899	0.276	NA	NA	NA
N-stage
N0	1			1
N1-2	2.511	1.468-4.295	0.001	2.149	1.226-3.767	0.008
M-stage
M0	1			1
M1	11.737	5.442-25.313	< 0.001	10.907	4.937-24.096	< 0.001
Tumor differentiation
Moderate/good	1
Poor	1.140	0.537-2.418	0.733	NA	NA	NA
Vascular invasion
No	1
Yes	3.246	1.874-5.625	< 0.001	NS	NS	NS
Tumor location
Rectum	1
Colon	1.557	0.810-2.992	0.184	NA	NA	NA
CEA level (ng/mL)
≤ 5	1
> 5	2.958	1.692-5.172	< 0.001	NS	NS	NS
Twist2 expression
Negative	1			1
Positive	2.632	1.184-5.809	0.017	3.264	1.455-7.375	0.004

Multivariate analysis included adjustment for N-stage, M-stage, vascular invasion, serum carcinoembryonic antigen level and Twist2 expression. T: Tumor; N: Node; M: Metastasis; HR: Hazard ratio; NA: Not available; NS: Not significant.