Hepatitis B virus induces expression of cholesterol metabolism-related genes via TLR2 in HepG2 cells

Figure 4 Effects of toll-like receptor 2-SiRNA on the expression of proteins related to cholesterol metabolism and intake of cholesterol by HepG2 cells treated with adenovirus hepatitis B virus. A: The protein expression of low-density lipoprotein receptor (LDLR) and 3-hydroxy-3-methylglutharyl-coenzyme A reductase (HMGCoAr) were assayed; B: HepG2 Cells were exposed for 4 h to Dil-acl-LDL (10 μg/mL), after thorough washing with phosphate buffered solution, fluorescence of Dil-acl-LDL was detected in cytoplasm of cells by fluorescence microscopy; C: Relative quantity of Dil-acl-LDL in HepG2 cells were analyzed by software. Results were representative of three similar experiments. ^aP < 0.05, ^bP < 0.01 vs control group. TLR2: Toll-like receptor 2; LDLR: Low-density lipoprotein receptor; LDL: Low density lipoprotein; Ad-HBV: Adenovirus hepatitis B virus.