miRNA-338-3p suppresses cell growth of human colorectal carcinoma by targeting smoothened

Figure 3 miRNA-338-3p regulates expression of smoothened in SW-620 cells. A: Smoothened (SMO) 3’-untranslated region potentially targeted by miRNA-338-3p (miR-338-3p) as predicted by TargetScan; B: Western blotting analysis showing SMO protein expression in SW-620 cells. β-actin was used as a housekeeping gene to normalize SMO protein expression. Lane 1: blank control; lane 2: SW-620 cells transduced with lentivirus pLV-THM-control; lane 3: SW-620 cells transduced with lentivirus pLV-THM-miR-338-3p; lane 4: SW-620 cells transduced with lentivirus pLV-THM-miR-338-3p-inhibitor. The results are representative of three independent experiments.