Role of T cell death in maintaining immune tolerance during persistent viral hepatitis

Figure 9 Figure illustrated the FACSan^® dot plots and histograms from peripheral blood lymphocytes from two hepatitis C virus patients with different CD127 expression on hepatitis C virus-specific cytotoxic T lymphocytes (CD8+/Pentamer+ cells). The different plots show the Bcl-2 interacting mediator (Bim) and myeloid cell leukemia sequence-1 (Mcl-1) expression directly ex vivo and after specific stimulation on peripheral CD8+/pentameter+ cells according to CD127 level. The figure on the top of the dot-plots represents the frequency of penatmer+ cells out of total CD8+ cells. The figure in the upper right corner in the histogram plot represents the MFI for Bim and Mcl-1 staining. The continuous and dashed line in the dot-plots and histograms represents the cut-off point to consider a staining positive according to the negative control. ND: Not done due to lack of pentamer+/CD8+ proliferation after specific stimulation.