Effects of small interfering RNA inhibit Class I phosphoinositide 3-kinase on human gastric cancer cells

Figure 6 Effects of adenovirus Class I phosphoinositide 3-kinase-RNA interference-green fluorescent protein on light chain 3 and Beclin-1 protein expression/Bcl-2 and p53 protein expression in SGC7901 cells. A: Effect of adenovirus Class I phosphoinositide 3-kinase [PI3K(I)]-RNA interference-green fluorescent protein (RNAi-GFP) (50 MOI) and adenovirus negative control (NC)-RNAi-GFP on light chain 3 (LC3) and Beclin-1 protein expression. Control: Adenovirus NC-RNAi-GFP. SGC7901 cells were treated with adenovirus PI3K(I)-RNAi-GFP (50 MOI) for 24 to 72 h then harvested for the extraction of total proteins. Adenovirus PI3K(I)-RNAi-GFP up-regulates the expression of LC3 and Beclin protein; B: Adenovirus PI3K(I)-RNAi-GFP (50 MOI) and adenovirus NC-RNAi-GFP on Bcl-2 and p53 protein expression. Control: Adenovirus NC-RNAi-GFP. SGC7901 cells were treated with adenovirus PI3K(I)-RNAi-GFP (50 MOI) for 24 to 72 h then harvested for the extraction of total proteins. Adenovirus PI3K(I)-RNAi-GFP up-regulates the expression of p53 and down-regulates the expression of Bcl-2 protein.