Effects of small interfering RNA inhibit Class I phosphoinositide 3-kinase on human gastric cancer cells

Figure 4 Microtubule-associated protein 1 light chain 3 expression and location in MGC803 cells after treatment with adenovirus Class I phosphoinositide 3-kinase-RNA interference-green fluorescent protein. Cells were treated with adenovirus Class I phosphoinositide 3-kinase [PI3K(I)]-RNA interference-green fluorescent protein (RNAi-GFP) (50 MOI) for 24 h (C), 48 h (D), and 72 h (E), and analyzed with an immunofluorescence microscope. A: Control; B: Adenovirus negative control-RNAi-GFP (× 400) (n = 3). Adenovirus PI3K(I)-RNAi-GFP increased the punctate distribution of light chain 3 from 24 to 72 h.