Effects of small interfering RNA inhibit Class I phosphoinositide 3-kinase on human gastric cancer cells

Figure 3 Monodansylcadaverine staining showed autophagy was activated after adenovirus Class I phosphoinositide 3-kinase-RNA interference-green fluorescent protein (50 MOI) treatment. A-D: SGC7901 cells; E-I: MGC803 cells incubated with adenovirus Class I phosphoinositide 3-kinase [PI3K(I)]-RNA interference-green fluorescent protein (RNAi-GFP) (50 MOI) and adenovirus negative control (NC)-RNAi-GFP for the indicated time and stained with monodansylcadaverine (100 μmol/L). Fluorescence particles showed L-acoustic vector sensor. A and E: Control; B and F: Adenovirus NC-RNAi-GFP; C and G: 24 h after adenovirus PI3K(I)-RNAi-GFP (50 MOI) treatment (× 200) (n = 3); D and H: 48 h after adenovirus PI3K(I)-RNAi-GFP (50 MOI) treatment; I: 72 h after adenovirus PI3K(I)- RNAi-GFP (50 MOI) treatment (× 1000) (n = 3).