Endoplasmic reticulum stress sensitizes human esophageal cancer cell to radiation

Figure 5 The effect of autophagy inhibition on cell apoptosis. EC109 cells were treated with tunicamycin (TM), radiation, and 3-MA for 24 h. A: The proportion of annexin V positive cells was measured and analyzed on a FACSort; B: Protein levels of GRP94, cleaved Caspase-3, and its substrate poly ADP-ribose polymerase (PARP) were assessed by Western blotting; C: EC109 cells were transfected with Beclin-1 siRNA. Two days after transfection, protein levels of Beclin-1 and ATG5 were determined by Western blotting; D, E: Western blotting of cells transfected with siRNA and treated with TM. Levels of LC3, ATG5, C/EBP homologous protein (CHOP), cleaved Caspase-3, and PARP were shown. N: Sham-treated with TM; R: 10 Gy irradiation; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.