Endoplasmic reticulum stress sensitizes human esophageal cancer cell to radiation

Figure 3 Apoptosis is enhanced by tunicamycin in irradiated cells. EC109 cells were pretreated with or without tunicamycin (TM) (5 μg/mL), followed by a single dose of 10 Gy. A: Western blotting analysis for poly ADP-ribose polymerase (PARP), Caspase-3, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH); B: Caspase-8 activity of treated cells; C: Apoptosis was determined by annexin V labeling; D: Representative images of cells stained with Hoechst 33343. N: Sham-treated with TM; R: 10 Gy irradiation.