Heme status affects human hepatic messenger RNA and microRNA expression

Figure 1 Heat maps with hierarchical clustering of mRNAs (A, C) and microRNAs (B, D) in Huh-7 cells exposed to heme, 10 μmol/L (yellow bars, left side of each panel) or 4, 6-dioxoheptanoic acid, 500 μmol/L (green bars, right side of each panel). A and B: The duration of exposure to heme or DHA was 6 h; C and D: The duration of exposure was 24 h. Cells were cultured, treated, harvested, and RNA arrays performed and analyzed as described in Materials and Methods. Blue color indicates decreased expression; red color indicates increased expression.