Effects of ginsenoside Rh2 on growth and migration of pancreatic cancer cells

Figure 4 Effects of Rh2 on proteins and mRNA expression of Bax, Bcl-2, survivin, cleaved caspase-3, caspase-8, caspase-9 in Bxpc-3 cells. A, B: Western blotting was used to examine protein expression of Bax, Bcl-2, survivin, cleaved caspase-3, caspase-8 and caspase-9. Rh2 upregulated protein expression of Bax, cleaved caspase-3 and caspase-9 in a dose-dependent manner and downregulated protein expression of Bcl-2 and survivin in a dose-dependent manner. However, Rh2 had no effect on protein expression of cleaved caspase-8; C, D: Reverse transcriptase-polymerase chain reaction was used to examine the expression of Bax, Bcl-2 and survivin mRNA. Rh2 (45 μmol/L) upregulated mRNA expression of Bax, and downregulated mRNA expression of Bcl-2 and survivin, ^aP < 0.05 vs 0 μmol /L group.