Mitofusin-2 ameliorates high-fat diet-induced insulin resistance in liver of rats

Figure 3 High-fat diets inhibited insulin pathway in liver of rats. Rats were fed with high-fat diets (HF) or normal control diets (Con) for 4 or 8 wk. A: The mRNA expression levels of mitofusin 2 (MFN2), insulin receptor (INSR), insulin receptor substrate 2 (IRS2), phosphoinositide-3-kinase (PI3K), AKT2 and glucose transporter type 2 (GLUT2) were examined by quantitative real-time-polymerase chain reaction; B and C: The protein levels were detected by Western-blotting. ^bP < 0.01 vs normal diets for 4 wk; ^dP < 0.01 vs normal diets for 8 wk.