Scolopendra subspinipes mutilans protected the cerulein-induced acute pancreatitis by inhibiting high-mobility group box protein-1

Figure 7 Effect of Scolopendra subspinipes mutilans on activation of mitogen activated protein kinases and nuclear factor-κB. A: Isolated pancreatic acinar cells were pretreated with Scolopendra subspinipes mutilans (SSM) for 1 h and then stimulated with cerulein for indicated times. The expression of phosphospecific mitogen activated protein kinases and degradation of Iκ-Bα in pancreatic acini was examined via western blot; B: The acinar cells were pretreated with c-Jun NH₂-terminal kinase (JNK) inhibitor (SP600125 50 μmol), p38 inhibitor (SB239063 20 μmol), nuclear factor-κB inhibitor (N-acetyl cystein, NAC, 10 mmol) or SSM (0.5 mg/mL) for 1 h. Then cerulein was added into isolated pancreatic acinar cells and incubated for 24 h. High-mobility group box protein-1 (HMGB-1) expression was measured via western blot. The results were similar in 3 further experiments. ERK: Extracellular signal-related kinase.