Scolopendra subspinipes mutilans protected the cerulein-induced acute pancreatitis by inhibiting high-mobility group box protein-1

Figure 6 Effect of Scolopendra subspinipes mutilans on cerulein-induced acinar cell death and the production of inflammatory mediators. The acinar cells were pretreated with Scolopendra subspinipes mutilans (SSM) for 1 h at indicated doses. A: 6 h after cerulein stimulation, the cell viability was measured; B, C: After 24 h of cerulein stimulation, cytokine levels in isolated pancreatic acinar cells were examined using enzyme-linked immunosorbent assay (B) and real-time reverse transcription polymerase chain reaction (C); D: And also the high-mobility group box protein-1 (HMGB-1) levels were measured by western blot. Data are represented as mean ± SE (n = 6 in each group). The results were similar in 3 further experiments. ^aP < 0.05 vs control group, ^cP < 0.05 vs cerulein treatment alone. TNF: Tumor necrosis factor; IL: Interleukins.