Mitofusin-2 ameliorates high-fat diet-induced insulin resistance in liver of rats

doi:10.3748/wjg.v19.i10.1572

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Mar 14, 2013 (publication date) through Dec 2, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 14, 2013; 19(10): 1572-1581
Published online Mar 14, 2013. doi: 10.3748/wjg.v19.i10.1572

Table 1 Primer sequences for quantitative polymerase chain reaction

Gene	bp	Forward primer (5'-3')	Reverse primer (5'-3')	GenBank No.
GAPDH	120	TGAACGGGAAGCTCACTGG	GCTTCACCACCTTCTTGATGTC	NM_017008
MFN2	160	AGCGTCCTCTCCCTCTGACA	TTCCACACCACTCCTCCGAC	NM_130894
INSR	135	TTTGCCCAACCATCTGTAAG	GACCATCCAGGTAGAAGTTTCG	NM_017071.1
IRS2	81	TCTCTGGCAGTTCAGGTCG	AGTCCTCTGGGTAAGGGTTG	NM_001168633
PI3K	135	GCCTGCTCTGTAGTGGTAGATG	GGAGGTGTGTTGGTAATGTAGC	NM_013005.1
AKT2	79	CTGAGATGATGGAGGTAGCG	CCGAGGAGTTTGAGATAATCG	NM_017093.1
GLUT2	80	AGCACATACGACACCAGACG	CAGACAGAGACCAGAGCATAGTG	NM_012879
SOCS3	148	TCACCCACAGCAAGTTTCC	ACGGCACTCCAGTAGAATCC	NM_053565.1

GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; MFN2: Mitofusin-2; INSR: Insulin receptor; IRS2: Insulin receptor substrate 2; PI3K: Phosphoinositide-3-kinase; AKT2: Protein kinase beta; GLUT2: Glucose transporter type 2; SOCS3: Suppressor of cytokine signaling 3.

Citation: Gan KX, Wang C, Chen JH, Zhu CJ, Song GY. Mitofusin-2 ameliorates high-fat diet-induced insulin resistance in liver of rats. World J Gastroenterol 2013; 19(10): 1572-1581
URL: https://www.wjgnet.com/1007-9327/full/v19/i10/1572.htm
DOI: https://dx.doi.org/10.3748/wjg.v19.i10.1572