S100A4 silencing blocks invasive ability of esophageal squamous cell carcinoma cells

Figure 4 S100A4 overexpression promotes invasive capability of TE13 cells. A: Transmigration cells of control, mock cDNA, S100A4 cDNA and S100A4 cDNA + E-cadherin cDNA cells was calculated from three independent experiments. The indicated cells (1 × 10⁴) were seeded on 8-mm porous transwell chambers. After 24 h of plating, transmigration cells were fixed and stained with crystal violet. Transmigration cells were counted for each of the indicated cells. Columns, mean number of cells obtained in three independent experiments; bars, SD; ^aP < 0.05 vs other 3 groups respectively; B: Western blotting, analysis for S100A4 protein expression; C: Western blotting analysis for E-cadherin protein expression.