S100A4 silencing blocks invasive ability of esophageal squamous cell carcinoma cells

Figure 3 Knockdown of S100A4 inhibits invasive capability of EC109 cells. A: Transmigration cells of control, mock siRNA, S100A4 siRNA and S100A4 siRNA + S100A4 cDNA cells was calculated from three independent experiments. The indicated cells (1 × 10⁴) were seeded on 8-mm porous transwell chambers. After 24 h of plating, transmigration cells were fixed and stained with crystal violet. Transmigration cells were counted for each of the indicated cells. Columns, mean number of cells obtained in three independent experiments; bars, SD; ^aP < 0.05 vs control or mock siRNA group; B: Western blotting, reverse transcription-polymerase chain reaction (RT-PCR) and quantitative real time PCR (Q-PCR) analysis for S100A4 and mRNA and protein expression, ^bP < 0.05 vs control or mock siRNA group; C: Western blotting, RT-PCR and Q-PCR analysis for E-cadherin and mRNA and protein expression. ^cP < 0.05 vs S100A4 siRNA group. 1: Control; 2: Mock siRNA; 3: S100A4 siRNA; 4: S100A4 siRNA + E-cadherin siRNA.