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World J Gastroenterol. Feb 28, 2012; 18(8): 754-766
Published online Feb 28, 2012. doi: 10.3748/wjg.v18.i8.754
Published online Feb 28, 2012. doi: 10.3748/wjg.v18.i8.754
Figure 6 Over-expression of resistin-like molecule β decreased the expression of vascular endothelial growth factor, but not v-ets erythroblastosis virus E26 oncogene homolog 1, in gastric cancer cells.
A: Western blotting indicated that 72 h after transfection, over-expression of resistin-like molecule β (RELMβ) abolished the expression of vascular endothelial growth factor (VEGF), but not v-ets erythroblastosis virus E26 oncogene homolog 1 (Ets1), in SGC-7901 and MKN-45 cells. Moreover, transfection of empty vector (mock) did not influence the expression of VEGF and Ets1; B: Reverse transcription polymerase chain reaction (RT-PCR) indicated the decreased VEGF transcription levels in SGC-7901 and MKN-45 cells transfected with pcDNA3.1-RELMβ for 72 h, but not in mock group. Moreover, the Est1 transcription levels were not influenced by transfection of pcDNA3.1-RELMβ or empty vector (mock); C: Real-time quantitative RT-PCR further demonstrated that transfection of pcDNA3.1-RELMβ for 72 h resulted in decreased transcription levels of VEGF, but not of Ets1, in SGC-7901 and MKN-45 cells. The symbol (a) indicates a significant decrease from parental cells (P < 0.01). Triplicate experiments were performed with essentially identical results. GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.
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Citation: Zheng LD, Yang CL, Qi T, Qi M, Tong L, Tong QS. Effects of resistin-like molecule β over-expression on gastric cancer cells
in vitro . World J Gastroenterol 2012; 18(8): 754-766 - URL: https://www.wjgnet.com/1007-9327/full/v18/i8/754.htm
- DOI: https://dx.doi.org/10.3748/wjg.v18.i8.754