Germline mutation analysis of MLH1 and MSH2 in Malaysian Lynch syndrome patients

Table 2 List of primer sequences used for polymerase chain reaction amplification of the exons of MSH2 gene and their respective sizes of polymerase chain reaction products

MSH2 gene	Primer sequence (5’-3’)	Size (bp)	Ta (°C)
Exon 1	5’ gtctgcttatgattggttgc 3’	632	58
	5’ actccgtgatcacaagttca 3’
Exon 2	5’ gtggaactataagtccagtaagc 3’	457	50
	5’ cagccaaactgcaacttttt 3’
Exon 3	5’ agaatcgattgaacccttga 3’	554	62
	5’ gtcaattaaagagcctttcc 3’
Exon 4	5’ acatcatatcagtgtcttgcac 3’	302	50
	5’ taatccatgtacctgattctcc 3’
Exon 5	5’ gaaggaacaccaaggaaaat 3’	273	50
	5’ ctgaaaaaggttaagggctctg 3’
Exon 6	5’ ggtcttaggaagaggaactttt 3’	337	50
	5’ gtataatcatgtgggtaactgc 3’
Exon 7	5’ ttgagacttacgtgcttagttg 3’	391	50
	5’ tctgaatgtgtcctaagagtga 3’
Exon 8	5’ aactttggagacctactgtact 3’	353	50
	5’ ccacaaaggtgctacaattag 3’
Exon 9	5’ tacatcatcagcactgtaactg 3’	494	53
	5’ ttgacagagatgtgaagtcatc 3’
Exon 10	5’ cagctttcaagtcagaaact 3’	511	53
	5’ aagaaagcttgactcttacctg 3’
Exon 11	5’ tcacgtagtacacattgcttct 3’	365	61
	5’ tatgtgcaagagtaacttccag 3’
Exon 12	5’ caaatggggggattaaatgt 3’	377	50
	5’ aaaacgttacccccacaaag 3’
Exon 13	5’ gcagtaactctgtccacatctt 3’	432	62
	5’ cttctcacaggacagagacata 3’
Exon 14	5’ atgtttgtggcatatccttc 3’	499	50
	5’ cagactgtgaattaaggggtaa 3’
Exon 15	5’ ctaatgacaaggtgagaaggat 3’	338	50
	5’ ccttcatcttagtgtcctgttt 3’
Exon 16A	5’ ggaactagacagtgcacacata 3’	428	62
	5’ ctgggatttttcacgtagtaac 3’
Exon 16B	5’ attattcaggagttcctgtcc 3’	500	50
	5’ ctctgagctatttgtttcttcc 3’

bp: Base pair; Ta: Annealing temperature.